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兔基质金属蛋白酶9Elisayabo官网手机版 盒,BR
Rabbit MMP-9 Elisa Kit
(订货以英文名为准)
产品编号:AC16954
品牌:Acmec
中文名称:
兔基质金属蛋白酶9Elisayabo官网手机版
盒
英文名称:
Rabbit MMP-9 Elisa Kit
保存条件:
2-8℃
中文名称: 兔基质金属蛋白酶9检测yabo官网手机版
盒
英文名称: Rabbit MMP-9 ELISA Kit
别名: 92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase
储存条件: 2-8℃,6 months
规格: 96T ; 48T
检测目标: MMP-9
样品类型: 血清/血浆/细胞培养上清
反应性: Rabbit
应用: ELISA
反应类型: Sandwich-ELISA
定量/定性: 定量检测
检测范围: 12.5-800 pg/ml
灵敏度: 5 pg/ml
特异性: 不与ApoAI,BMP1,BMP2,BMP3,BMP4,BMP7,CRP,HGF,HSP27,IL-1α,IL-1RI,IL-1β,IL-1RA,IL-2,IL-4,IL-5,IL-6,IL-8,IL-10,IL-12,IL-13,IL-15,IL-17C,IL-21,IL-23,MMP-2,sIL-2R,sIL-6R,PDGF,PLA2G7,prolactin,TGFβ1,TGFβ2,TGFβ3,TLR1,TLR2,TLR3,TNF-α,TNFRI,TNFRII,VEGF等反应。
精密度: 板内,板间变异系数均<10%
回收率: 80%-120%
反应时间: 2.33h Swiss Prot: P41246
Gene ID: 100008993
检测原理: Acmec ELISAyabo官网手机版 盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗兔MMP-9单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的兔MMP-9会与酶标板上的包被抗体充分结合;洗板后加入生物素化抗兔MMP-9抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的兔MMP-9发生特异性结合;洗板后加入辣根过氧化物酶(HRP)标记的链霉亲和素,生物素与链霉亲和素会发生高强度的非共价结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的兔MMP-9,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的兔MMP-9浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中兔MMP-9的浓度。
背景说明: The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP). 暂无数据 1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。
英文名称: Rabbit MMP-9 ELISA Kit
别名: 92 kDa gelatinase; 92 kDa type IV collagenase; CLG4B; Gelatinase B; GELB; macrophage gelatinase; MANDP2; matrix metallopeptidase 9; matrix metalloproteinase-9; MMP9; MMP-9; type V collagenase
储存条件: 2-8℃,6 months
规格: 96T ; 48T
检测目标: MMP-9
样品类型: 血清/血浆/细胞培养上清
反应性: Rabbit
应用: ELISA
反应类型: Sandwich-ELISA
定量/定性: 定量检测
检测范围: 12.5-800 pg/ml
灵敏度: 5 pg/ml
特异性: 不与ApoAI,BMP1,BMP2,BMP3,BMP4,BMP7,CRP,HGF,HSP27,IL-1α,IL-1RI,IL-1β,IL-1RA,IL-2,IL-4,IL-5,IL-6,IL-8,IL-10,IL-12,IL-13,IL-15,IL-17C,IL-21,IL-23,MMP-2,sIL-2R,sIL-6R,PDGF,PLA2G7,prolactin,TGFβ1,TGFβ2,TGFβ3,TLR1,TLR2,TLR3,TNF-α,TNFRI,TNFRII,VEGF等反应。
精密度: 板内,板间变异系数均<10%
回收率: 80%-120%
反应时间: 2.33h Swiss Prot: P41246
Gene ID: 100008993
检测原理: Acmec ELISAyabo官网手机版 盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗兔MMP-9单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的兔MMP-9会与酶标板上的包被抗体充分结合;洗板后加入生物素化抗兔MMP-9抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的兔MMP-9发生特异性结合;洗板后加入辣根过氧化物酶(HRP)标记的链霉亲和素,生物素与链霉亲和素会发生高强度的非共价结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的兔MMP-9,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的兔MMP-9浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中兔MMP-9的浓度。
背景说明: The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP). 暂无数据 1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。
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