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大鼠脂联素ELisayabo官网手机版 盒,BR
Rat Adiponectin ELISA KIT
(订货以英文名为准)
产品编号:AC16892
品牌:Acmec
中文名称:
大鼠脂联素ELisayabo官网手机版
盒
英文名称:
Rat Adiponectin ELISA KIT
保存条件:
2-8℃
中文名称: 大鼠脂联素检测yabo官网手机版
盒
英文名称: Rat Adiponectin ELISA Kit
别名: ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28apM1; Gelatin-binding protein
储存条件: 2-8℃,6 months
规格: 96T ; 48T
检测目标: Adiponectin
样品类型: 血清/血浆/细胞培养上清
反应性: Rat
应用: ELISA
反应类型: Sandwich-ELISA
定量/定性: 定量检测
检测范围: 43.75-2800 pg/ml
灵敏度: 21.75 pg/ml
特异性: 不与大鼠Adiponectin类似物等反应。
精密度: 板内,板间变异系数均<10%
回收率: 80%-120%
反应时间: 3.5h
Gene ID: 246253
检测原理: Acmec ELISAyabo官网手机版 盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠Adiponectin单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的大鼠Adiponectin会与酶标板上的包被抗体充分结合;洗板后加入生物素化抗大鼠Adiponectin抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠Adiponectin发生特异性结合;洗板后加入辣根过氧化物酶(HRP)标记的链霉亲和素,生物素与链霉亲和素会发生高强度的非共价结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的大鼠Adiponectin,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的大鼠Adiponectin浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠Adiponectin的浓度。
背景说明: Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin and adiponectin protein of 30 kDa. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, mRNA levels of Adiponectin have been shown to be induced over 100-fold. Studies indicate that Adiponectin enhances the ability of sub-physiological levels of insulin to suppress glucose production, thus linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol-3-kinase (PI3-K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. mRNA levels of Adiponectin have been shown to be significantly reduced in adipose tissue of obese patients with Type 2 diabetes relative to control subjects. Structural similarities to TNFalpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Because Adiponectin is down-regulated in various forms of obesity and its structural similarity to TNFalpha, it is currently being investigated as an important regulator of whole body energy homeostasis. 暂无数据 1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。
英文名称: Rat Adiponectin ELISA Kit
别名: ACDC; Acrp30; ACRP30ADPN; adipocyte, C1Q and collagen domain containing; AdipoQ; ADIPQTL1; apM-1; APM1APM-1; C1q and collagen domain-containing protein; GBP28apM1; Gelatin-binding protein
储存条件: 2-8℃,6 months
规格: 96T ; 48T
检测目标: Adiponectin
样品类型: 血清/血浆/细胞培养上清
反应性: Rat
应用: ELISA
反应类型: Sandwich-ELISA
定量/定性: 定量检测
检测范围: 43.75-2800 pg/ml
灵敏度: 21.75 pg/ml
特异性: 不与大鼠Adiponectin类似物等反应。
精密度: 板内,板间变异系数均<10%
回收率: 80%-120%
反应时间: 3.5h
Gene ID: 246253
检测原理: Acmec ELISAyabo官网手机版 盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠Adiponectin单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的大鼠Adiponectin会与酶标板上的包被抗体充分结合;洗板后加入生物素化抗大鼠Adiponectin抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠Adiponectin发生特异性结合;洗板后加入辣根过氧化物酶(HRP)标记的链霉亲和素,生物素与链霉亲和素会发生高强度的非共价结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的大鼠Adiponectin,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的大鼠Adiponectin浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠Adiponectin的浓度。
背景说明: Adipose cells produce and secrete numerous physiologically important proteins, such as lipoprotein lipase (LPL), leptin and adiponectin protein of 30 kDa. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation, mRNA levels of Adiponectin have been shown to be induced over 100-fold. Studies indicate that Adiponectin enhances the ability of sub-physiological levels of insulin to suppress glucose production, thus linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol-3-kinase (PI3-K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. mRNA levels of Adiponectin have been shown to be significantly reduced in adipose tissue of obese patients with Type 2 diabetes relative to control subjects. Structural similarities to TNFalpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Because Adiponectin is down-regulated in various forms of obesity and its structural similarity to TNFalpha, it is currently being investigated as an important regulator of whole body energy homeostasis. 暂无数据 1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。
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