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人胰岛素样生长因子结合蛋白7检测yabo官网手机版 盒,BR
Human IGFBP-7 ELISA KIT
(订货以英文名为准)
产品编号:AC16518
品牌:Acmec
中文名称:
Human IGFBP-7 ELISA KIT
英文名称:
Human IGFBP-7 ELISA KIT
保存条件:
2-8℃
中文名称: 人胰岛素样生长因子结合蛋白7检测yabo官网手机版
盒
英文名称: Human IGFBP-7 ELISA Kit
别名: angiomodulin; FSTL2; IBP-7; IGFBP7; IGFBP-7v; IGFBPrp1; IGFBP-rp1; insulin-like growth factor binding protein 7; MAC25 protein; Prostacyclin-stimulating factor; PSF; PSFAGM; TAF; Tumor-derived adhesion factor
储存条件: 2-8℃,6 months
规格: 96T ; 48T
检测目标: IGFBP-7
样品类型: 血清/血浆/细胞培养上清
反应性: Human
应用: ELISA
反应类型: Sandwich-ELISA
定量/定性: 定量检测
检测范围: 15.62-1000 pg/ml
灵敏度: 7.8 pg/ml
特异性: 不与人IGF-I,人IGF-IR,人IGF-II,人IGFBP-1,人IGFBP-2,人IGFBP-3,人IGFBP-4,人IGFBP-5,人IGFBP-6,小鼠IGFBP-rp1等反应。
精密度: 板内,板间变异系数均<10%
回收率: 80%-120%
反应时间: 3h 检测原理: Acmec ELISAyabo官网手机版 盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人IGFBP-7单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的人IGFBP-7会与酶标板上的包被抗体充分结合;洗板后加入HRP酶标记的抗人IGFBP-7抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的人IGFBP-7发生特异性结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的人IGFBP-7,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的人IGFBP-7浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中人IGFBP-7的浓度。
背景说明: The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. ALS (Acid Labile Subunit) is a liver-derived protein that exists in a ternary complex with Insulin-like Growth Factor (IGF)-binding Protein-3 (IGFBP-3) or IGFBP-5, and either IGF-I or IGF-II. ALS increases the half-life of IGF/IGFBP complexes in circulation. 暂无数据 1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。
英文名称: Human IGFBP-7 ELISA Kit
别名: angiomodulin; FSTL2; IBP-7; IGFBP7; IGFBP-7v; IGFBPrp1; IGFBP-rp1; insulin-like growth factor binding protein 7; MAC25 protein; Prostacyclin-stimulating factor; PSF; PSFAGM; TAF; Tumor-derived adhesion factor
储存条件: 2-8℃,6 months
规格: 96T ; 48T
检测目标: IGFBP-7
样品类型: 血清/血浆/细胞培养上清
反应性: Human
应用: ELISA
反应类型: Sandwich-ELISA
定量/定性: 定量检测
检测范围: 15.62-1000 pg/ml
灵敏度: 7.8 pg/ml
特异性: 不与人IGF-I,人IGF-IR,人IGF-II,人IGFBP-1,人IGFBP-2,人IGFBP-3,人IGFBP-4,人IGFBP-5,人IGFBP-6,小鼠IGFBP-rp1等反应。
精密度: 板内,板间变异系数均<10%
回收率: 80%-120%
反应时间: 3h 检测原理: Acmec ELISAyabo官网手机版 盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人IGFBP-7单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的人IGFBP-7会与酶标板上的包被抗体充分结合;洗板后加入HRP酶标记的抗人IGFBP-7抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的人IGFBP-7发生特异性结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的人IGFBP-7,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的人IGFBP-7浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中人IGFBP-7的浓度。
背景说明: The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF. ALS (Acid Labile Subunit) is a liver-derived protein that exists in a ternary complex with Insulin-like Growth Factor (IGF)-binding Protein-3 (IGFBP-3) or IGFBP-5, and either IGF-I or IGF-II. ALS increases the half-life of IGF/IGFBP complexes in circulation. 暂无数据 1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。
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